Comparison of two processing techniques on the sperm quality of semen contaminated with urine.

Urospermia in stallions can occur intermittently, consistently, or as an isolated event, and may result in reduced sperm quality which is often assumed to reduce fertility. Although sperm quality declines in urospermic ejaculates, fertility has not been assessed in mares bred with urine contaminated semen. The aims of this study were to compare sperm quality after simple dilution (SD), cushioned centrifugation (CC) alone, or cushioned centrifugation combined with a 40 % silane-coated silica solution (SC) in semen contaminated with 0, 20, or 40 % (v/v) urine. Sperm quality values tended to decrease as the percent urine increased within all treatments (SD, CC, SC) after 24 h of cooled storage. However, SC treated groups had higher sperm quality compared to SD and CC when exposed to 20 or 40 % (v/v) urine. Differences in pregnancy rates among treatment groups (SD with 0 or 40 % (v/v) urine, or 40 % (v/v) urine followed by SC) were unable to be detected.

Impact of Equine and Bovine Oocyte Maturation in Follicular Fluid From Young and Old Mares on Embryo Production in Vitro.

Equine follicular fluid (FF) provides autocrine and paracrine factors from theca, granulosa, and cumulus cells, both reflecting and impacting oocyte and follicle maturation. We hypothesized that maturation of oocytes in FF from old versus young mares has a deleterious effect on oocyte maturation and their subsequent developmental potential. Follicular fluid was collected from the large, dominant follicle from young mares (4-13 years) or old mares (21-26 years) and classified as: (1) Noninduced follicular fluid (NFF), FF from noninduced follicle 33 ± 3 mm, or (2) Induced follicular fluid (IFF), FF collected ∼24 hours after administration of ovulation-inducing drugs when a follicle 33 ± 3 mm was observed. In experiment 1, immature equine oocytes were collected, matured in vitro for 30 ± 2 hours in 100% IFF, collected from young or old mares, with the addition of follicle stimulating hormone (5 mU/mL), then fertilized by intracytoplasmic sperm injection. In experiment 2, immature bovine oocytes were collected, matured in 100% IFF or NFF, collected from young mares or old mares, then fertilized via in vitro fertilization. In experiment 1, more blastocysts tended (P = .08) to be produced from equine oocytes that were matured in old versus young mare FF. In experiment 2, when IFF and NFF groups were combined, cleavage rates were higher (P = .001) when bovine oocytes were matured in FF from young than old mares. In contrast to our hypothesis, we observed no conclusive evidence that FF from old mares has a deleterious impact on oocytes and their early developmental potential.